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KMID : 0903519710140020131
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1971 Volume.14 No. 2 p.131 ~ p.135
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Studies on the Degradation of L - Tyrosine by Aspergillus oryzae
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Abstract
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1. L-Tyrosine-α-ketoglutaric transaminase and p-hydroxyphenylpyruvic acid oxidase are distributed in Aspergillus oryzae.
2. L-Tyrosine oxidation in extracts of acetone powder, cell free extract and culture liquid of Aspergillus oryzae cultivated in the shaking culture are considerably accelerated by the addition of α-ketoglutaric acid and then formation of glutamic acid was identified by chromatography method.
3. The roles of α-ketoglutaric acid and pyridoxal phosphate have been shown to be an amino group acceptor in a transamination reaction.
4. Enzyme systems of an extracts of acetone powder and cell free extract also rapidly oxidized L-tyrosine and p-hydroxyphenlpyruvic acid to homogentisic acid.
5. The optimum pH for L-tyrosine-α-ketoglutaric acid transaminase was pH values of 6.0 and 6.5, and that for p-hydroxyphenylpyruvic acid oxidase was at pH values of 7.5.
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